Anupalli Roja Rani
Osmania University, India
Title: Anti-proliferative activity and HER2 receptor expression analysis of MCF-7 (breast cancer cell) cells by plant extract coleusbarbatus (Andrew)
Biography
Biography: Anupalli Roja Rani
Abstract
Among several, breast cancer has emerged as the most common female cancer in developing countries. It is the most common cause of cancer related deaths worldwide among women.It is a molecularly and clinically heterogeneous disease. Moreover, it is a hormone – dependent tumor in which estrogens can regulate the growth of breast cells by binding with estrogen receptors (ERs) moreover, the use of natural products in cancer therapeutics are due to their properties of bio compatibility and less toxicity. Plants are the vast reservoirs for various bioactive compounds. Coleus barbatus (Lamiaceae) contains anticancer property against several cancer cell lines.
In the present study, an attempt is being made to enrich the knowledge of anticancer activity of pure compounds extracted from Coleus barbatus (Andrew). on human breast cancer cell lines MCF-7. Here in, we are assessing the antiproliferative activity of Coleus barbatus (Andrew) plant extracts against MCF 7 and also evaluating their toxicity in normal human mammary cell line such as HMEC. The active fraction of plant extract was further purified with the help of Flash chromatography, MPLC and preparative HPLC. The structure of pure compound will be elucidated by using modern spectroscopic method like NMR, ESI-MS methods. Later, the growth inhibition morphological assessment of cancer cells and cell cycle analysis of purified compound assessed using FACS. Growth and progression of signaling molecules HER2, GRP78 studied by secretion assay using ELISA and expression analysis by flow cytometry.
Cytoxoxic effect against MCF-7 with IC50 values were derived from doseresponse curves, using six concentrations of twofold serially diluted samples, by SOFTMax Pro software (Molecular device) and respectively Ellipticine and 0.5% DMSO were used as positive and negative control.
The present study shows the significance of various bio-active compounds extracted from Coleus barbatus (Andrew) root material. It acts as an anti-proliferative and shows cytotoxic effect on human breast cancer cell lines MCF7.The plant extracts play an important role pharmacologically. The whole plant has been used in traditional medicine for decades and the studies done have authentcated the practice. Earlier as describe the plant have been used in the ayurveda and homeopathy medicine. However more clinical and pathological studies must be conducted to investigate the unexploited potential of the plant. These studies are very useful for drug designing in future.