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Nora Alkeraishan

King Saud university Riyadh, Saudi Arabia

Title: The effect of melatonin and Vitamin D3 on the gene expression of P53 in MCF-7 breast cancer cell line

Biography

Biography: Nora Alkeraishan

Abstract

P53 was identified as first tumor suppressor gene which is actively involved in numerous cellular mechanisms such as initiating DNA repair mechanisms, apoptosis and cell cycle arrest. More than 50% of all human cancers have a mutated non-functional p53 expression. Breast cancer (BC) is one of the leading cause of mortality in females and mutated p53  is documented to be the causitive agent in only 20% of them. However, mutation in p53 in BC results in more aggresive form of cancer which is more resistant to the conventional therapies. Recently, multiple clinical trials suggested that the combined use of melatonin and vitamin D3 can slow down the growth of breast cancer cells. The genetic and molecular mechanisms through which these compounds initiate the cancerous cells to apoptosis or cell cycle arrest is  still not fully understood. This study aims to investigate the effect of melatonin, vitamin D3 and their combined  treatment on the proliferation of breast cancer cell line MCF-7 by MTT assay. Our results showed that melatonin, vitamin D3 and combined effect of vitamin D3+ melatonin inhibit proliferation of these cells by upregulating gene and protein expression of p53.