Biography:

Dr. Hongying He completed her PhD in microbiology at State University of New York at Buffalo in 2000 and her MD at Albert Einstein College of Medicine in New York City in 2005. She completed her radiology residency in the McGovern Medical School at UT Health in 2010 and her breast imaging fellowship at M.D. Anderson Cancer Center in 2011. She is currently the Chief of Breast Imaging in the Department of Diagnostic and Interventional Imaging at the McGovern Medical School. Her main research interest includes correlating imaging features of breast cancer with underlying biology and clinical outcomes

Abstract:

Objective: To compare the incremental cancer detection rate (ICDR) using bilateral whole-breast ultrasonography (BWBUS) versus dynamic contrast-enhanced magnetic resonance imaging (MRI) in patients with primary breast cancer.

Methods: A retrospective database search in a single institution identified 259 patients with breast cancer diagnosed from January 2011 through August 2014 who underwent mammography, BWBUS, and MRI before surgery. Patient characteristics, tumor characteristics, and lesions seen on each imaging modality were recorded. The sensitivity, specificity, and accuracy for each modality were calculated. ICDRs according to index tumor histology and receptor status were also evaluated. The effect of additional cancer detection on surgical planning was obtained from the medical records.

Results: A total of 266 additional lesions beyond 273 index malignancies were seen on at least one modality, of which 121 (45%) were malignant and 145 (55%) benign. MRI was significantly more sensitive than BWBUS (p=0.01), while BWBUS was significantly more accurate and specific than MRI (p<0.0001). Compared with mammography, the ICDRs using BWBUS and MRI were significantly higher for estrogen receptor-positive and triple-negative cancers, but not for human epidermal growth factor receptor-2-positive cancers. Twenty-two additional malignant lesions in 18 patients were seen on MRI only. Surgical planning remained unchanged in eight (44%) of those 18 patients.

Conclusion: MRI was more sensitive than BWBUS, while BWBUS was more accurate and specific than MRI. MRI-detected additional malignant lesions did not change surgical planning in almost half of these patients. BWBUS may be a cost-effective and practical tool in breast cancer staging.

Biography:

Zhygulin A V is a Physician, general and oncological surgeon, breast and reconstructive surgeon. He is the Founder and Chief of the first in Ukraine regular twice a year two-week intensive educational course of Oncoplastic and reconstructive surgery and modern breast cancer treatment for the breast surgeons, Kyiv, Ukraine and Founder of Ukainian International breast conference, Kyiv, Ukraine (2014, 2015 and 2016). He has been serving as a Head of Breast Unit in LISOD Israeli Cancer Care Hospital, Kyiv, Ukraine, the first in Ukraine appropriate EUSOMA criteria (Full Membership of Breast Centers Network since 2013) since 2011. He is the Member of ESSO

Abstract:

Introduction: Breast conserving surgery (BCS) followed by radiation therapy and skin-sparing mastectomy with immediate reconstruction (IR) have proven their efficiency and safety. The technical and oncological aspects of breast oncoplastic surgery in our breast unit, organized according EUSOMA criteria are presented.

Materials & Methods: Treatment was carried out on multidisciplinary tumorboard. During BCS we used therapeutic mammoplasties or flaps from the chest, abdominal wall, LICAP-flaps or LD-flaps. In case of mastectomy we offered immediate reconstruction. Type of reconstruction was chosen according clinical situation. Photos of the patients were performed.

Results: From 2007 to 09.2016 1032 surgeries in 715 patients were performed. 400 oncoplastic BCS were performed in 392 patients. Average age was 53.2(22-88), tumor size – 2,15cm (0, 8-15), weight of specimen - 109g (6-1034). We used therapeutic mammaplasties in 140 (35.0%) cases, local chest wall flaps - in 93 (23.3%). Complications had 102(25.5%) patients. We followed up 342 (87.2%) patients during 35.5 (6-108) months. Local recurrence occurred in 8(2.3%) patients. 35(10.2%) patients had metastasis, 18(5.3%) of them died. In IR group 148 immediate reconstructions were performed in 123 patients. The average age was 45 (29-71).We did 68 (45.9%) one-stage reconstructions, 26(17.5%) - with ADM, 17(11.5%) - with synthetic mesh. Two-stage surgeries were done in 56(37.8%) cases. LD-flap was done in 9(6.8%) patients, LD-flap + implant - in 11(7.4%), pedicled TRAM-flap - in 4(2.7%). Skin-sparing mastectomy was performed in 30(20.3%) patients, skin-reducing - in 38(25, 7%), nipple-sparing in 55(37,2%). We followed up 103(83.7%) patients during 32 months (6-84). Local recurrence was found in 1(0.9%) patient, distant metastases - in 17(16.5%) and 6(5.8%) of them died. Early complications were found in 51(34.4%) cases.

Conclusions: We evaluated our experience in oncoplastic BCS and reconstructive surgery in a dedicated BU that would allow for radical treatment with excellent aesthetical outcomes.

Biography:

Uhi Toh has completed his MD and PhD from Kurume University School of Medicine. He is currently working as the Associate Professor of Department of Surgery and the Director of Breast Surgery of Kurume University Hospital. He has published more than 50 papers in reputed journals

Abstract:

Background: The sentinel lymph nodes status of breast cancer is used to predict the status of the remaining axillary lymph nodes accurately and sentinel lymph node biopsy (SLNB) provide staging information and to determine the need for axillary lymph node dissection in breast cancer patients. The purpose of this study was to evaluate the utility of a color charge-coupled device (CCD) camera system for the intraoperative detection of SLNs and to determine its clinical efficacy and sensitivity in patients with operable breast cancer.

Methods: The intraoperative detection of SLNs was performed using the conventional Indigo Carmine dye (indigotindisulfonate sodium) technique combined with a new Indocyanine green (ICG) imaging system (HyperEye Medical System: HEMS, MIZUHO IKAKOGYO, Japan) to map SLNs, in which the lymphatic vessels and SLNs were visualized transcutaneously with illuminating ICG fluorescence. One intradermal injection of 3.5ml indigo carmine (blue dye) mixed with 0.5ml indocyanine green (ICG) was performed via the subareolar plexus followed by 5 minutes breast massage for dilating breast lymphatics. After an inferior axillary incision, the search of SLN was guided by HEMs for lighting stained lymphatic channels leading to blue-stained LNs.

Results: Between January 2012 and May 2013, SLNs were successfully identified in all 168 patients (detection rate: 100%). By histopathology, the sensitivity was 93.8% for the detection of the metastatic involvement of SLNs (15 of 16 nodal-positive patients). Immunohistology revealed one false-negative case among 16 patients (6.3%). After a median follow-up of 30.5 months, none of the patients presented with axillary recurrence.

Conclusion: These results suggest that the HEMS imaging system is a feasible and effective method for the detection of SLNs in breast cancer. Furthermore, the HEMS device permitted the transcutaneous visualization of lymphatic vessels under light conditions, thus facilitating the identification and detection of SLNs without affecting the surgical procedure, together with a high sensitivity and specificity.

Biography:

Marike Gabrielson has completed her PhD in experimental breast cancer studies in 2013 from the University of Örebro, Sweden, and is currently conducting Postdoctoral studies at Karolinska Institutet in Stockholm, Sweden. She is part of the KARMA study scientific board. She is also Project Manager of tissue collection and experimental work with the overarching goal to identify protein and hormone markers of mammographic density and breast cancer risk.

Abstract:

Following age, mammographic density (MD) is considered one of the strongest risk factors for breast cancer. Despite the association between MD and breast cancer risk, little is known about the underlying histology and biological basis of breast density. To better understand the mechanisms behind MD we conducted large-scale proteomic analyses of blood plasma using two sample sets comprising 729 and 600 women, and assessed morphology, proliferation and hormone receptor status through immunohistochemical staining in breast tissues from 160 women. Plasma and tissue protein expressions and morphology was assessed in relation to absolute area-based breast density. All samples were collected from non-diseased women as part of the KARMA (Karolinska mammography project for risk prediction for breast cancer) project. The KARMA study is a population-based prospective cohort with the overarching goal to reduce the incidence and mortality of breast cancer by focusing on individualized prevention and screening. Plasma profiling revealed 20 proteins with linear associations to MD. These proteins have previously been described in processes of tissue homeostasis, DNA repair, cancer development and/or progression in MD. In breast tissue, high MD was associated with higher amount of stroma and epithelium and less amount of fat, but was not associated with a change in epithelial proliferation or receptor status. Increased expression of both epithelial progesterone receptors and stromal oestrogen receptors was associated with a greater proportion of stroma, suggesting hormonal involvement in regulating breast tissue composition. Our current data is indicative of the mechanistic processes underlying MD and provide insights into the aetiology of MD as a prominent risk factor for breast cancer.

Biography:

Jana Slobodnikova has done her MD from Prague Charles University. She has completed CSc/PhD from Institus of experimental Oncology Slovac Scientic Academy. She also worked as an Ass. Prof. Trnaviensis University. She has published more than 90 scientific papers, from then 25 papers in reputed journals and has been serving as an Editorial Board Member of repute. She has published 3 monographs. She is President of the section of breast imaging of Slovak Radiologic Society and Vice President of the Slovak Society of Ultrasound in Medicine.

Abstract:

Breast cancer is the most common malignancy of the female population the incidence is increasing mainly statistically between 50s and 60s, 60s and 70s. Recently, however, we meet more often with the occurrence of breast cancer in women in 30s and significantly between 20 and 40 year. For women this age range is not exist preventive or screening mammography, in Slovakia only sonography and only clinical self- examination by touch. The period from 1.5.2005 and 30.6.2016 we performed more than 70000 mammography examinations and more than 185000 ultrasound examinations. The youngest patient was 6 months, the oldest 94 years. Patients were sent for examination by attending gynecologist, general practitioner. Preventive examinations completed asymptomatic women without clinical symptoms. Young women and girls were examined by ultrasound with 18 MHz linear proge, with CFM and elastography. Next if necessary, we performed mammography, MR-mammography a CCB too. During the monitored period we diagnosed 398 new cases of the breast cancer, all cases are verified by histopathology. The age distribution of patients with newly diagnosed cancer we transparently stored in tables and graphs. We focused on women in the age group to 39 and 49 year old. In category women between 18s and 39s we diagnosed 32 new cases of breast cancer, between 40s and 49s 64 cases. Summary are 86 of new cases of the breast cancer in women between 18 and 49 years. We analyzed the different findings, especially with respect to possibility of diagnostic self-examination, combined with ultrasound, MR mammography, mammography and core cut biopsy under ultrasound control. The number of the young women with new diagnosed breast cancer slowly increased. Our aim is the early diagnostics, without vascular cancer vascular invasivity, without the lymphadenopathy. We retrospectively focused on genetic anamnesis factor, short time of the diagnosis and therapy.

Biography:

Hale Aydin has graduated from Ankara University School of Medicine, Ankara and completed residency from Baskent University School of Medicine Ankara (Turkey). She is a Radiologist with sub-specialty expertise in breast imaging, and she has been serving as breast Radiologist at Dr Abdurrahman Yurtaslan Ankara Oncology Training and Research Hospital, Ankara. Her research interests are focused on the use of imaging methods on breast cancers.

Abstract:

Imaging Findings of a Rare Aggressive Breast Cancer Subtype: Metaplastic Carcinoma of the Breast (E-MPBC): Metaplastic breast carcinoma (MPBC) is one of the rarest and generally poorly differentiated invasive breast carcinomas. According to our knowledge MPBC tend to show more benign imaging features, such as round, lobular, oval shape with a predominantly circumscribed contour. We analyzed 65 patients with pathologically proven MPBC between 2004 and 2016. We reviewed patients’ medical files and breast imaging records of the all patients. Patients ranged in age from 25 to 88 years (median, 53 years) and 16 patients were younger than 40 years-old. The most common mammographic findings were round shape, micro lobulated margin and high density masses. The most frequent sonographic presentations of MPBC were round shape, angular margin which partially indistinct, hypoechoic and heterogeneous echo pattern and no posterior feature masses. MRI was performed in 7 patients, and all lesions were presented as masses rather than non-mass enhancements. Six of the lesions showed irregular or speculated margins. Signal intensity-time curves were type-II or type-III. DWI was obtained in 3 patients and the lesions showed diffusion restriction in 2 patients. In conclusion, well-recognizing the findings of MPBC is very important. Because, according to previously reported series this aggressive, high grade tumor can display more benign imaging feature. This appearance can cause misdiagnosis as benign breast lesion especially in young women. Although the lesions demonstrate benign or moderately malign feature, MPBC should be kept in mind in differential diagnosis for large palpable breast masses.

Biography:

Alicia González González is a PhD student from Cantabria University School of Medicine. Currently, she is involved in breast cancer research and melatonin, specifically in the sensitizing effects of melatonin to chemotherapy and radiotherapy for its antiangiogenic and antiadipogenic actions. She has published 2 papers and has presented her work in 5 congress

Abstract:

Enhancing the radiosensitivity of cancer cells is one of the most important goals in clinical radiobiology. Melatonin exerts oncostatic effects on breast cancer by reducing estrogen biosynthesis in human breast cancer cells, surrounding fibroblasts and endothelial cells and by regulating cytokines that influence tumor microenvironment. This hormone also has antiangiogenic activity in tumoral tissue. Vascular endothelial growth factor (VEGF) produced from tumor cells is essential for the expansion of breast cancer. Thus, the aim of the present study was to investigate whether melatonin sensitizes endothelial cells to radiotherapy by regulating angiogenesis and estrogen biosynthesis. To accomplish this we used cocultures of HUVEC cells with MCF-7 cells. The expression of genes was analyzed by RT-PCR. Cell proliferation was measured by the MTT method, the migration of HUVECs was measured by the wound healing assay and tubulogenesis studies were performed in a tubulogenesis multiplate system in vitro. Only the presence of malignant epithelial cells in the cocultures altered proliferation, the expression of genes involved in the local biosynthesis of estrogens and VEGF, in endothelial cells. In addition, ionizing radiation decreased cell proliferation and VEGF expression, and melatonin pretreatment 1 mM led to a significantly greater decrease. Furthermore, the migration of endothelial cells and the tube formation were reduced with the radiation and melatonin pretreatment resulted in a significantly higher reduction. Our results demonstrate that melatonin could exert a cooperative enhancement of radiosensitivity associated with the modulation of angiogenesis and local estrogen biosynthesis

Biography:

Tong Li is a PhD candidate at Faculty of Health Sciences, The University of Sydney. Her PhD project is studying breast density and cancer presentations for women in China. This project will be the first study to determine the optimum image technology for policy makers in China who are considering the implementation and implications of a national population-based breast cancer screening program. She published a literature review regarding breast cancer in China last year in Breast Cancer Research and Treatment with an impact factor of 4.

Abstract:

Breast density (BD) is an independent risk factor for breast cancer but its characteristics are poorly understood in China. This study aims to identify associated factors with BD in Chinese women by using a quantitative method. 84 cancer and 987 cancer-free women were recruited from Fudan University Shanghai Cancer Centre. BD was measured by an automatic algorithm autodensity and expressed in both percentage density (PD) and dense area (DA) format. Pearson tests were performed to exam relationships between density and continuous variables and t-tests were conducted to compare differences of mean density values for categorical variables. Multivariate models were built using variables being statistically significant from the Pearson and t-tests. For cancer-free women, weight and BMI were negatively associated (r=-0.237, r=-0.272) with PD whereas positively associated (r=-0.495, r =-0.520) with DA; age was associated with PD (r=-0.202) and DA (r=-0.086) but did not add any prediction within multivariate models. Lower PD was found within women with secondary education background or below compared to women with tertiary education. For cancer-women, PD showed similar relationships with that of cancer-free women whilst breast area was the only factor that was associated with DA (r=0.739). This is the first time that BD was measured by a quantitative method for Chinese women and important associations were identified for both PA and DA. The findings are very valuable to policy makers being responsible for introducing effective models of breast cancer prevention and diagnosis

Biography:

Liu Yan was born in Shandong province, China in 1989. He obtained B.S in Bioscience from Northeast Agriculture University in 2012. Now he is studying PhD degree in southeast University major in oncobiology

Abstract:

Invasive breast cancer is the leading cause of women mortality. Protein kinase D2 (PRKD2) and PRKD3 but not PRKD1, were implicated to positively contribute to invasive breast cancer growth and progression. In current study, we found that PRKD2 and PRKD3 function as important oncogenic drivers in invasive breast cancer with evidences showing that PRKD2 and PRKD3 were preferentially expressed in invasive breast cancer cells and tissues to promote breast cancer growth in vitro and in vivo. To uncover the molecular mechanisms of PRKD2 and PRKD3 in invasive breast cancer, phosphoproteome, interactome and transcriptome of PRKD2 and PRKD3 were systematically investigated. Besides identification of PRKD2 and PRKD3 regulated phosphoproteins, interacting proteins and target genes expression, 36 hub nodes including known breast cancer drivers, such as TP53, MYC and BRCA1, were identified in PRKD2 and PRKD3 regulating networks. ELAVL1 and UBC were recognized as the most common hub nodes across PRKDs networks. The enriched pathway analysis reveals that PRKD2 and PRKD3 regulated pathways contribute to multiple cancer related events, including cell cycle, apoptosis, migration, angiogenesis, cancer energy metabolism and cancer immunity. Enrichment of cell cycle and cell mobility related pathways across PRKDs networks, explained the observations that depletion of PRKD2 or PRKD3 or both or inhibition of PRKDs activity led to alternation of cancer cell cycle and decrease of cancer cell migration ability. Besides common features, notable variations were also observed from phosphoproteome, interactome to transcriptome between PRKD2 and PRKD3, indicating that PRKD2 and PRKD3 have shared specific functions and mechanisms. Finally, our studies raised promising invasive breast cancer therapeutic drug targets, such as ELAVL1, Ubiquitin system, HDACs and so on. Taken together, the important roles as well as the molecular mechanisms of PRKD2 and PRKD3 in invasive breast cancer were uncovered to provide clues for further combating invasive breast cancer.

Biography:

Nguyen Thu Huong is a MD radiologist in Bach Mai hospital of Vietnam, PhD student in Gunma University of Japan.

Abstract:

Objective: To evaluate the potential of ¹⁸F-FDG PET and diffusion weighted MR Imaging (DWI) in predicting response of triple negative breast cancer (TNBC) to Cisplatin treatment. Methods: Cisplatin (10 mg/kg) was injected one shot intraperitoneally into TNBC tumor bearing mice. Animals were imaged on PET and MRI scanners dedicated to animal use before treatment (day 0) and days 3 and 7 after treatment. The highest standardized uptake value (SUVmax) and the average of apparent diffusion coefficient value (ADCmean) were measured. Results: We evaluated tumor growth of the non-treated mice (n=8) and treatment mice. Treated mice were divided into the response group (n=7) and the no response group (n=7) based on whether the  tumor growth was similar to or slower than that of the non-treated mouse. SUVmax value on day 3 and day 7 (SUV3, SUV7) showed significant difference between the response group and no response group (P<0.05), however, ratio of SUVmax on day 7 to day 0 (SUV7/0) showed no significant diffrence between three groups (P> 0.05). ADCmean value on day 0 (ADC0) of the response group was significantly lower than that of the no response group (P<0.01). The ratio of ADCmean on day 7 to day 0 (ADC7/0) showed significant difference between three groups (P<0.01), and ADC7/0 of the response group were significantly higher than those of control group and no response group. Conclusions: ADC0, ADC7/0 are potential early predictors of response of TNBC to Cisplatin treatment.